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SynRT

SynRT provides robust reverse transcriptase activity – with a 65°C optimal temperature – being the most thermotolerant reverse transcriptase in the market.

 

SynRT
Catalog # Size Price
R001M50 reaction160 €
R001L200 reaction500 €

Properties

SynRT provides robust reverse transcriptase activity – with a 65°C optimal temperature – being the most thermotolerant reverse transcriptase in the market. The enzyme retains its highly efficient reverse transcriptase activity throughout the entire cDNA synthesis, generates the highest yields of cDNA and is also capable to synthesize long RNA samples.  SynRT is the engineered large fragment of Bacillus stearothermophilus DNA polymerase and it’s not only capable of highly efficient full-length cDNA synthesis, but it can handle a wide range of sample types, especially the ones with secondary structures that would be hard to reverse transcribed at lower temperatures.

Figure 1. Comparing the efficiency and specificity of SynRT with a leading competitor’s reverse trancriptase in an experiment under the same conditions.

Suggested reaction conditions

Using SynRT reverse transcriptase  DNA synthesis can be performed at a constant temperature. The recommended temperature is 65 °C, but the enzyme works well in broad ranges of temperature (55-70 °C) and can be activated by heat in 80°C.

Contents

R001M 10,000 U (200 U/µl) = 50 x 25 µl reactions

Material provided: SynRT enzyme 10.000 U (50 µl), 10x SynRT Buffer (1.5 ml) and 100 mM MgSO4 solution (1.5 ml), 100 µM Oligo(dT)18 (60 µl), and 100 µM Random Hexamer (60 µl)
R001L 40,000 U (200 U/µl) = 200 x 25 µl reactions

Material provided: SynRT enzyme 40,000 U (200 µl), 10x SynRT Buffer (1.5 ml) and 100 mM MgSO4 solution(1.5 ml),100 µM Oligo(dT)18 (240 µl), and 100 µM Random Hexamer (240 µl)

Storage

The enzyme preserves its condition up to 6 months in room temperature. For a longer period of time store at -20°C.

Documents

Manual for SynRT

SDS for SynRT

SDS for SynRT MM

FAQ

RNA purity and integrity are essential for cDNA synthesis. Always use freshly prepared RNA. It is not recommended to use multiple frozen RNA sample.

Using SynBst cDNA synthesis is the most effective at higher temperatures.

• Use certified reagents, including enzyme-free water • Use an RNase inhibitor to protect RNA sample • Change gloves frequently • Sterilise the tools to be used for cDNA synthesis

The cDNA can be stored at -20°C for up to one week or -80°C for longer storage.

Why choose us

Our products cover the most commonly used enzymes in molecular biology, such as high fidelity and strand displacement DNA polymerase and reverse transcriptase with a unique enhanced room temperature stability.

CONTACT US

Contact Info

  • info@synthbiotics.com

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