SynRT provides robust reverse transcriptase activity – with a 65°C optimal temperature – being the most thermotolerant reverse transcriptase in the market. The enzyme retains its highly efficient reverse transcriptase activity throughout the entire cDNA synthesis, generates the highest yields of cDNA and is also capable to synthesize long RNA samples. SynRT is the engineered large fragment of Bacillus stearothermophilus DNA polymerase and it’s not only capable of highly efficient full-length cDNA synthesis, but it can handle a wide range of sample types, especially the ones with secondary structures that would be hard to reverse transcribed at lower temperatures.
Figure 1. Comparing the efficiency and specificity of SynRT with a leading competitor’s reverse trancriptase in an experiment under the same conditions.
Suggested reaction conditions
Using SynRT reverse transcriptase DNA synthesis can be performed at a constant temperature. The recommended temperature is 65 °C, but the enzyme works well in broad ranges of temperature (55-70 °C) and can be activated by heat in 80°C.
|R001M||10,000 U (200 U/µl) = 50 x 25 µl reactions
Material provided: SynRT enzyme 10.000 U (50 µl), 10x SynRT Buffer (1.5 ml) and 100 mM MgSO4 solution (1.5 ml), 100 µM Oligo(dT)18 (60 µl), and 100 µM Random Hexamer (60 µl)
|R001L||40,000 U (200 U/µl) = 200 x 25 µl reactions
Material provided: SynRT enzyme 40,000 U (200 µl), 10x SynRT Buffer (1.5 ml) and 100 mM MgSO4 solution(1.5 ml),100 µM Oligo(dT)18 (240 µl), and 100 µM Random Hexamer (240 µl)
The enzyme preserves its condition up to 6 months in room temperature. For a longer period of time store at -20°C.