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SynPhusion

SynPhusion DNA polymerase is a high efficiency DNA polymerase that possesses 50x higher fidelity than the traditionally used Taq polymerase. The polymerase is highly recommended for GC rich sequences, long templates, high-throughput PCRs, NGS library generation, mutagenesis and other cloning methods where high fidelity is a must.

SynPhusion MM
Catalog # Size Price
P003S100U120 €
P003M500U460 €
P003L2000U1950 €

SynPhusion
Catalog # Size Price
P001S100U70 €
P001M500U315 €
P001L2000U1190 €

Properties

SynPhusion DNA polymerase is a high efficiency DNA polymerase that possesses 50x higher fidelity than the traditionally used Taq polymerase. The polymerase is highly recommended for GC rich sequences, long templates (up to 20 kb), high-throughput PCRs, NGS library generation, mutagenesis and other cloning methods where high fidelity is a must. SynPhusion DNA Polymerase also possesses 3´→ 5´ exonuclease activity and generates blunt-ended products.

SynPhusion Gold & SynPhusion Gold Master Mix’s buffers contain two tracking dyes and a density reagent for directly loading PCR results onto a gel. The density reagent and dyes work well with downstream applications including DNA sequencing, ligation, and restriction digestion without interfering with the SynPhusion DNA polymerases’ exceptional performance.

SynPhusion Master Mix & SynPhusion Gold Master Mix contains the SynPhusion High-Fidelity DNA Polymerase enzyme, SynPhusion Buffer/ SynPhusion Gold Buffer and also dNTP all in one tube. Using these Master Mixes for PCR can be very beneficial as it eliminates the need to measure and add individual components each time an experiment is performed.

Figure 1.  This PCR result is a typical sample of comparing the efficiency and specificity of the SynPhusion DNA polymerase and High Fidelity Polymerase enzymes of leading comptetitors’ under the same conditions.

SynPhusion polymerase is not only more efficient in amplification thus generating much more specific product, but has increased fidelity next to leading competitor enzymes.

Figure 2. Amplification curves of SynPhusion and a leading competitor’s High Fidelity Polymerase after fourteen days of incubation at -20°C or at 25°C. This PCR result shows that our SynPhusion polymerase can amplify the DNA template without significant quality reduction after a 2 week incubation time at room temperature. Black amplification curves represent the SynPhusion polymerase’s reaction time which was kept at  -20°C at all times, green amplification curves represent the SynPhusion polymerase’s amplification time which was incubated at 25°C for 14 days and blue amplification curves represent the amplification time of a a leading competitor’s High Fidelity Polymerase which was kept at -20°C .

Our SynPhusion polymerase is able to last up to 6 months at room temperature without significant efficiency loss, ensuring that the polymerase doesn’t suffer any quality reduction during transport.

Contents

P001S 100 U (1 U/µl) = 100 x 50 µl reactions

Material provided: SynPhusion DNA Polymerase 100 U (100 µl), 10x SynPhusion DNA Polymerase Buffer (1.5 ml), and 100 mM MgSO4 solution (1.5 ml)
P001M 500 U (1 U/µl) = 500 x 50 µL reactions

Material provided: SynPhusion DNA Polymerase 500 U (500 µl), 10x SynPhusion DNA Polymerase Buffer (2 x 1.5 ml), and 100 mM MgSO4 solution (1.5 ml)
P001L 2000 U (1 U/ µl) = 2000 x 50 µl reactions

Material provided: SynPhusion DNA Polymerase 2000 U (2x 1 ml), 10x SynPhusion DNA Polymerase Buffer (10 x 1.5 ml), and 100 mM MgSO4 solution (5x 1.5 ml)
P003S 100 x 50 µl reactions

Material provided: 2x SynPhusion DNA Polymerase MM 100 U (2x 1250 µl), and 100 mM MgSO4 solution (1.5 ml)
P003M 500 x 50 µl reactions

Material provided: 2x SynPhusion DNA Polymerase MM 100 U (10x 1250 µl), and 100 mM MgSO4 solution (1.5 ml)
P003L 2000 x 50 µl reactions

Material provided: 2x SynPhusion DNA Polymerase MM 100 U (40x 1250 µl), and 100 mM MgSO4 solution (5x 1.5 ml)
P002S 100 U (1 U/µl)

Material provided: SynPhusion Gold DNA Polymerase 100 U (100 µl), 5x SynPhusion Gold DNA Polymerase Buffer (2 x 1.5 ml), and 100 mM MgSO4 solution (1.5 ml)
P002M 500 U (1 U/µl)

Material provided: SynPhusion DNA Polymerase 500 U (500 µl), 5x SynPhusion Gold DNA Polymerase Buffer (10 x 1.5 ml), and 100 mM MgSO4 solution (2x 1.5 ml)
P002L 2000 U (1 U/ µl)

Material provided: SynPhusion DNA Polymerase 2×1000 U (2×1000 µl), 5x SynPhusion Gold DNA Polymerase Buffer (30 x 1.5 ml), and 100 mM MgSO4 solution (5x 1.5 ml)
P004S 100 x 50 µl reactions

Material provided: 2x SynPhusion Gold MM 100 U (2x 1250 µl), and 100 mM MgSO4 solution (1.5 ml)
P004M 500 x 50 µl reactions

Material provided: 2x SynPhusion Gold MM 500 U (10x 1250 µl), and 100 mM MgSO4 solution (2x 1.5 ml)
P004L 2000 U x 50 µl reactions

Material provided: 2x SynPhusion Gold MM 2000 U (40x 1250 µl), and 100 mM MgSO4 solution (5x 1.5 ml)

Storage
The enzyme preserves its condition up to 6 months in room temperature. For a longer period of time store at -20°C.

 

Documents

Manual for SynPhusion

Manual for SynPhusion MM

Manual for SynPhusion Gold

Manual for SynPhusion Gold MM

SDS for SynPhusion

SDS for SynPhusion MM

SDS for SynPhusion Gold

SDS for SynPhusion Gold MM

FAQ

Blue SynPhusion includes dyes for direct loading of PCR products on a gel.

No

No. SynPhusion DNA Polymerase produces blunt-end products.

• Add DMSO to the final reaction (2-8%) • Use longer denaturation time • Try 2 step PCR

• Repeat the PCR and make sure that there are no pipetting errors. • Use primers with Tm 60°C or higher. Tm values can be increased by lengthening the primers. Preferable primer length is 20 nt or longer • Use fresh high quality dNTPs. Do not use dNTP mix that contains dUTP. • Sample concentration may be too low. Use more template. • Template DNA may be damaged. Use carefully purified template. • Lengthen extension time. • Increase the number of cycles. • Lower annealing temperature. • Optimize enzyme concentration. • Titrate DMSO (2-8 %) in the reaction. • Denaturation temperature may be too low. Optimal denaturation temperature for most templates is 98°C or higher. • Denaturation time may be too long or too short. Optimize denaturation time. • Check the purity and concentration of the primers. • Check primer design.

If you have high molecular weight smears: • Reduce enzyme concentration. • Shorten extension time. • Reduce the total number of cycles. • Increase annealing temperature or try 2-step protocol. • Vary denaturation temperature. • Optimize Mg2+ concentration. • Lower primer concentration. If you have low molecular weight discrete bands: • Raise annealing temperature. • Shorten extension time. • Lower enzyme concentration. • Optimize Mg2+ concentration. • Titrate template amount. • Lower primer concentration. • Design new primers.

Why choose us

Our products cover the most commonly used enzymes in molecular biology, such as high fidelity and strand displacement DNA polymerase and reverse transcriptase with a unique enhanced room temperature stability.

CONTACT US

Contact Info

  • info@synthbiotics.com

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