SynLD is a 6X DNA loading dye that contains three different dyes for visual tracking of DNA migration during electrophoresis. It can be used to prepare DNA samples for loading on agarose gels. The dyes have a slight negative charge and migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. It enables the tracking of the migration of samples without the use of UV light. The product also allows the sample to form a layer at the bottom of the well without glycerol. The rate of migration varies with gel composition. It can be diluted to 1:5 with sample before loading and the rate of migration will vary with the composition of gel.
Figure 1. The vibrant three colour ensure the DNA migration tracking during the process of agarose gel electrophoresis, represented in a standard 1% gel run in SB buffer.
Figure 2. Enhanced UV evaluation. SynLD does not interfere with the evaluation of results creating almost undetectable black marks during UV light.
Figure 3. The rate of migration varies with the composition of gel.
Suggested reaction conditions
Add 1/6 volume of 6X SynLD loading dye to the DNA sample, mix it gently before loading it on the agarose gel.
|L001M||5x 1.0 ml 6xSynLD|
|L001L||20x 1.0 ml 6xSynLD|
It can be stored at room temperature or at 4 ºC for periods up to 12 months. For a longer period of time store at -20°C.