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SynLD

SynLD is a 6X DNA loading dye that contains three different dyes for visual tracking of DNA migration during agarose electrophoresis.

 

SynLD
Catalog # Size Price
L001M5 x 1.0 mL30 €
L001L20 x 1.0 mL100 €

Properties

SynLD is a 6X DNA loading dye that contains three different dyes for visual tracking of DNA migration during electrophoresis. It can be used to prepare DNA samples for loading on agarose gels. The dyes have a slight negative charge and migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. It enables the tracking of the migration of samples without the use of UV light.  The product also allows the sample to form a layer at the bottom of the well without glycerol. The rate of migration varies with gel composition. It can be diluted to 1:5 with sample before loading and the rate of migration will vary with the composition of gel.

Figure 1. The vibrant three colour ensure the DNA migration tracking during the process of agarose gel electrophoresis, represented in a standard 1% gel run in SB buffer.

Figure 2. Enhanced UV evaluation. SynLD does not interfere with the evaluation of results creating almost undetectable black marks during UV light.

afdafFigure 3. The rate of migration varies with the composition of gel.

Suggested reaction conditions

Add 1/6 volume of 6X SynLD loading dye to the DNA sample, mix it gently before loading it on the agarose gel.

Contents

L001M 5x 1.0 ml 6xSynLD
L001L 20x 1.0 ml 6xSynLD

         

Storage

It can be stored at room temperature or at 4 ºC for periods up to 12 months. For a longer period of time store at -20°C. 

 

Documents

Manual for SynLD

SDS for SynLD

FAQ

The main function of DNA loading dye is to provide a dense solution that allows the DNA samples to sink to the bottom of the well and stay there during electrophoresis. Additionally, it contains 3 different dyes that enable visualizing the DNA band on the gel after running it.

It is recommended to mix the DNA loading dye at a ratio of 1:6 to (v/v) with the DNA sample.

This could be caused by incorrect gel percentage, incorrect staining protocol, or loading too much or too little of PCR products on gel. Or it may be a case of no amplification occured or poor quality of amplified product.

This can be caused by using a low concentration of DNA.

This can be caused by using a low percentage of agarose in the gel, or by poor gel casting technique.

This can be caused by poor quality of the DNA sample, or overloading the sample.