SynLAMP Mix works efficiently with multiple amplification inhibitory factors such as high GC content, secondary structures in template, and low template concentration. It includes all of the necessary components for the reaction, such as the buffer, enzymes, dye, dNTP in a single, pre-mixed solution. This makes it much easier and more efficient to set up experiments, as you don’t have to measure out each component every time you perform the reaction. The dye in the enzyme is an intercalating dye that can be used to track a LAMP reaction in real-time by fluorescence measurement. One of the enzymes is the SynBst that is the engineered large fragment of Bacillus stearothermophilus DNA polymerase, it has robust strand displacement activity and catalyses the 5’-3’ synthesis of DNA without detectable 3′-5′ exonuclease activity. The other enzyme, SynRT has a very effective reverse transcription activity, is capable to highly efficient full-length cDNA synthesis in a wide range of sample types up to 12 kb and does not contain RNaseH.
Figure 1. This loop mediated amplification reaction shows that our SynLAMP Mix can amplify the RNA template faster than a leading competitor’s LAMPMix. Green amplification curves represent the SynLAMP Mix’s reaction time, blue amplification curves represent a leading competitor enzyme.
Our SynLAMP Mix is able to last up to 6 months at room temperature without significant efficiency loss, ensuring that the enzymes doesn’t suffer any quality reduction during transport.
Figure 2. This loop mediated amplification reaction shows that our SynLAMP Mix can amplify the RNA template without significant quality reduction after a 2 week incubation time at room temperature. Blue amplification curves represent the SynLAMP Mix’s reaction time which was kept at the recommended -20°C at all times and green amplification curves represent the SynLAMP Mix’s amplification time which was incubated at 25°C for 14 days.
Suggested reaction conditions
Using SynLAMP Mix DNA synthesis can be performed at a constant temperature. The recommended temperature is 65 °C, but the enzymes work well in broad ranges of temperature (55-70 °C) and can be inativated by heat in 80°C.
|L011M||100 x 25 µL reactions
Material provided: 2x SynLAMP Mix (1250 µl), 100 mM MgSO4 solution (1.5 ml) and 50x LAMP Fluorescent Dye (50 µl)
|L011L||500 x 25 µl reactions
Material provided: 2x SynLAMP Mix (5x 1250 µl), 100 mM MgSO4 solution (5 x 1.5 ml) and 50x LAMP Fluorescent Dye (250 µl)
The enzymes preserve their condition up to 6 months at room temperature. For a longer period of time store at -20°C.