Sign in

SynBst

SynBst is the engineered large fragment of Bacillus stearothermophilus DNA polymerase. Our modified protein can reach 2,5 x faster amplification, has robust strand displacement activity, and catalyzes the 5’-3′ synthesis of DNA without detectable exonuclease activity.

SynBst MM
Catalog # Size Price
B002M1600 U75 €
B002L8000 U290 €

SynBst
Catalog # Size Price
B001M1600 U65 €
B001L8000 U250 €

Properties

Its fast amplification and very robust strand displacement activity make SynBst a perfect candidate for special PCR applications like isothermal amplification (LAMP), whole genome amplification (WGA), multiple displacement amplification (MDA). BstSyn also works efficiently with multiple factors such as high GC content, secondary structures in template, and low template concentration.

Using the SynBst Master Mix  (SynBst MM) can be very beneficial as it eliminates the need to measure and add individual components each time an experiment is performed. It includes all of the necessary components for the reaction, such as the enzyme, buffer, and deoxynucleoside triphosphates (dNTPs), in a single, pre-mixed solution. This makes it much easier and more efficient to set up experiments, as you don’t have to measure out each component every time you perform the reaction.

SynBst Polymerase is faster than competitor enzymes: our enzyme is able to significantly shorten the reaction time compared to a leading competitor’s Bst Polymerase.

Figure 1. This Loop mediated amplification reaction shows that our SynBst Polymerase can amplify the RNA template faster than a leading competitor’s Bst Polymerase. Green amplification curves represent the SynBst Polymerase’s reaction time, blue amplification curves represent a leading competitor enzyme.

SynBst  is able to last up to 6 months at room temperature without significant efficiency loss, ensuring that the polymerase doesn’t suffer any quality reduction during transport( Fig.2).

Figure 2. SynBst Polymerase amplification curves after fourteen days of incubation at -20°C or at 25°C. This Loop mediated amplification reaction shows that our SynBst Polymerase can amplify the RNA template without significant quality reduction after a 2 week incubation time at room temperature. Blue amplification curves represent the SynBst Polymerase’s reaction time which was kept at the recommended -20°C at all times and green amplification curves represent the SynBst Polymerase’s amplification time which was incubated at 25°C for 14 days.

Suggested reaction conditions
Using SynBst DNA synthesis can be performed at a constant temperature. The recommended temperature is 65 °C, but the enzyme works well in broad ranges of temperature (55-70 °C) and can be inactivated by heat in 80°C.

Contents

B001M 1600 U (8 U/µl) = 200 x 25 µl reactions

Material provided: SynBst Polymerase 1600 U (200 µl), 10x SynBst Polymerase Buffer (1.5 ml), and 100 mM MgSO4 solution (1.5 ml)
B001L 8000 U (8 U/µl) = 1000 x 25 µl reactions

Material provided: SynBst Polymerase 8000 U (1 mL), 10x SynBst Polymerase Buffer (2 x 1.5 ml), and 100 mM MgSO4 solution (2 x1.5 ml)
B002M 200 x 25 µl reactions

Material provided: 2x SynBst MM 1600 U (2x 1250 µl), and 100 mM MgSO4 solution (1.5 ml)
B002L 1000 x 25 µl reactions

Material provided: 2x SynBst MM 8000 U (10x 1250 µl), and 100 mM MgSO4 solution (5x 1.5 ml)

Storage
The enzyme preserves its condition up to 6 months in room temperature. For a longer period of time store at -20°C.

Documents

Manual for SynBst

Manual for SynBST MM

SDS for SynBst

SDS for SynBst MM

FAQ

SynBst Polymerase has a high resistance to high temperatures, which allows it to maintain its activity at the high temperatures used in PCR reactions. It also has a high processivity, meaning it can continue to extend a primer for an extended period of time without dissociating from the template DNA.

The optimal temperature range for SynBst Polymerase activity is between 60-70°C. However, it can remain active at temperatures up to 80°C.

Yes, SynBst Polymerase has been shown to have a high tolerance for GC-rich templates and can be used in PCR amplification of these types of DNA sequences.

SynBst Polymerase requires a template DNA, primers, deoxynucleoside triphosphates (dNTPs), and a buffer containing MgCl2 for optimal activity.

When using SynBst Polymerase in a multiplex PCR reaction, it's important to optimize the reaction conditions for the specific primer sets and template DNA sequences being used. As with any PCR reaction, it is also important to confirm the specificity of the amplified products by gel electrophoresis or sequencing.

This may be due to insufficient amounts of SynBst Polymerase or template DNA in the reaction, or incorrect reaction conditions (such as low magnesium concentration or incorrect annealing temperature).

This can be caused by the presence of impurities in the template DNA, or by the use of primers with low specificity.

This can be caused by the use of primers with similar sequences, or by the use of too high primer concentrations.

This may be due to low levels of dNTPs in the reaction or to an insufficient extension time.

Why choose us

Our products cover the most commonly used enzymes in molecular biology, such as high fidelity and strand displacement DNA polymerase and reverse transcriptase with a unique enhanced room temperature stability.

CONTACT US

Contact Info

  • info@synthbiotics.com

Menu

Quick Links

Copyright © 2022 All rights reserved.