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What PCR is

What PCR is Polymerase Chain Reaction (PCR) is a widely used molecular biology technique for amplifying DNA segments. However, PCR can be challenging when the DNA template has a high GC content. GC-rich DNA sequences can form secondary structures that hinder the binding of primers and the activity of the DNA polymerase. In this blog […]

Molecular cloning

Molecular cloning   Molecular cloning is a powerful tool in molecular biology that allows researchers to make copies of DNA fragments and insert them into vectors for further analysis or manipulation. There are several common techniques for molecular cloning, each with their own advantages and disadvantages. In this article, we’ll discuss the different techniques and […]

How to amplify GC rich regions?

How to amplify GC rich regions? Polymerase Chain Reaction (PCR) is a widely used molecular biology technique for amplifying DNA segments. However, PCR can be challenging when the DNA template has a high GC content. GC-rich DNA sequences can form secondary structures that hinder the binding of primers and the activity of the DNA polymerase. […]